Coding

Part:BBa_K1796008:Design

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)


nifD promoted from Paenibacillus sp. WLY78


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1116
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 150
    Illegal AgeI site found at 1141
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut site was involved after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.


Source

NifD(BBa_K1796008) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

References